Influenza A/B/Pandemic

Kits: EliGene® Influenza A/B/pandemic LC Kit (ref: 90058-LC)
Package size:
50 reactions
Analytical specificity: Influenza virus A, B, A/H1N1
Analytical sensitivity: 1 – 10 viruses in the amplified sample
Specimens: serum, plasma, sputum, bronchoalveolar lavage, nasopharyngeal swab, nasopharyngeal aspirate, tissue
Compatible instruments:
ABI 7300, ABI 7500 (FAST) (Applied Biosystems); LightCycler® 480 (Roche); RotorGene 6000 or RotorGene Q (Qiagen); MyGo Pro (IT-IS Life Science)
CE certification: yes
Detected DNA region: Genome
Detection technology: Molecular Beacons
Clinical study description and results:
Within the frame of testing functional characteristics of EliGene® Influenza A/B/Pandemic LC kit overall 100 clinical specimens were analyzed. From these specimens 61 blind specimens and 39 positive specimens were verified by Cepheid Xpert® Flu Kit and INFLUENZA A/B Q-PCR Alert kit. From these 100 samples 9 samples were Influenza A virus RNA positive, 6 samples Influenza B virus RNA positive and 24 Influenza A/H1N1 virus RNA positive. The EliGene® Influenza A/B/Pandemic LC kit diagnosed as Influenza A, B, A/H1N1 virus RNA positive all 39 specimens. There were no discrepant results. Totally 61 specimens were right determined by the EliGene® Influenza A/B/Pandemic LC kit as Influenza A, B, A/H1N1 RNA negative.
Sensitivity: 100 %
Specificity: 100 %
Detection technology description:
The EliGene® Influenzavirus A/B/H1N1 kit is intended for detection and quantification of Influenza A, B and H1N1 virus RNA. Kit contains reversion transcription in one mix with RealTime detection. Results of analyzes distinguishes Influenza A, B and H1N1 virus types.
Pathogen description:
Influenza is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The most common symptoms are chills, fever, runny nose, sore throat, muscle pains, headache, coughing, weakness and general discomfort. Approximately 33% of people with influenza are asymptomatic. Flu can occasionally lead to pneumonia, either direct viral pneumonia or secondary bacterial pneumonia, even for persons who are usually very healthy. Typically, influenza is transmitted through the air by coughs or sneezes, creating aerosols containing the virus. Influenza can also be transmitted by direct contact with bird droppings or nasal secretions, or through contact with contaminated surfaces.  Influenza viruses can be inactivated by sunlight, disinfectants and detergents. Often, new influenza strains appear when an existing flu virus spreads to humans from another animal species, or when an existing human strain picks up new genes from a virus that usually infects birds or pigs. An avian strain named H5N1 raised the concern of a new influenza pandemic after it emerged in Asia in the 1990s, but it has not evolved to a form that spreads easily between people. In April 2009 a novel flu strain evolved that combined genes from human, pig, and bird flu. Initially dubbed "swine flu" and also known as influenza A/H1N1. Influenza B almost exclusively infects humans. This type of influenza mutates at a rate 2–3 times slower than type A.
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