Kits: EliGene® Borrelia UNI Kit (ref: 90024-UNI) 
Package size:
50 reactions
Analytical specificity: Borrelia garinii, Borrelia afzelii and Borrelia burgdorferi sensu stricto
Analytical sensitivity: 1 – 10 copies of bacterial DNA in the amplified sample
Specimens: blood, urine, CSF, synovial fluid, tissue
Compatible instruments:
ABI 7000, ABI 7300, ABI 7500 (Applied Biosystems), LightCycler® 480 and LightCycler® Nano (ROCHE); fully compatible with RotorGene instruments; MyGo Mini, MyGo Pro (IT-IS Life Science)
CE certification: yes
Detected DNA region: genome (fla gene)
Detection technology: Molecular Beacons
Clinical study description and results:
Totally 486 clinical samples were tested by EliGene® Borrelia UNI Kit. EliGene® Borrelia UNI Kit detected correctly all 9 positive samples. Totally 477 samples were correctly diagnosed by EliGene® Borrelia UNI Kit as Borrelia negative.
Sensitivity: 100%
Specificity: 100%
Pathogen description:
Lyme borreliosis is a disease caused by spirochete Borrelia burgdorferi. The detection of this bacterium could be made from any clinical material using the DNA diagnostics in Europaen Union. There can be found three pathogenic species of Borrelia genus: B. b. sensu stricto, B. afzelii and B. garinii. EliGene® Borrelia Kits contain mix of primers and probes that are specific to all three mentioned Borrelia species. DNA diagnostics is useful in cases of Borrelia suspicion (anamnesis of tick or insect that suck the blood).
Hytönen J, Hartiala P, Oksi J, Viljanen MK. 2008. Borreliosis: recent research, diagnosis, and management. Scand J Rheumatol. 37(3):161-172.
Priem S, Rittig MG, Kamradt T, Burmester GR, Krause A. 1997. An optimized PCR leads to rapid and highly sensitive detection of Borrelia burgdorferi in patients with Lyme borreliosis. J Clin Microbiol. 35(3): 685–690.
Detection of pathogenic Borrelia sp. in DNA samples (isolated from ticks) by EliGene® Borelia UNI kit on ABI®7500FAST instrument.


Detection of pathogenic Borrelia sp. in DNA samples (isolated from ticks) by EliGene® Borelia UNI kit on ABI®7500FAST instrument.