EliGene - diagnostics of new generation
Special offer - sexually transmitted urinal infections...
We now offer a complete diagnostics set for the 4 sexually transmitted urinal infections. Chlamydia trachomatis, Neisseria gonorrhoeae, Ureaplasma sp., Mycoplasma hominis / genitalium
Borrelia diagnostics
| Kits: | EliGene® Borrelia LC Kit (ref: 90024-LC) EliGene® Borrelia RT Kit (ref: 90024-RT) |
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| Package size: |
50 reactions |
| Possibility of quantification: |
On demand |
| Analytical specificity: | Borrelia garinii, Borrelia afzelii and Borrelia burgdorferi sensu stricto |
| Analytical sensitivity: | 1 – 10 copies of bacterial DNA in the amplified sample |
| Specimens: | blood, urine, CSF, synovial fluid, tissue |
| Compatible instruments: |
EliGene® Borrelia LC Kit intended for LightCycler® 2.0, LightCycler 480, RotorGene 6000 EliGene® Borrelia RT Kit intended for ABI7000, ABI7300, ABI7500 instruments, fully compatible with RotorGene instruments |
| CE certification: | yes |
| Detected DNA region: | genome (fla gene) |
| Detection technology: | Molecular Beacons (LC version), TaqMan probes (RT version) |
| Clinical study description and results: | |
| Totally 486 clinical samples were tested by EliGene® Borrelia RT Kit. EliGene® Borrelia RT Kit detected correctly all 9 positive samples. Totally 477 samples were correctly diagnosed by EliGene® Borrelia RT Kit as Borrelia negative. Sensitivity: 100% Specificity: 100% |
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| Pathogen description: | |
| Lyme borreliosis is a disease caused by spirochete Borrelia burgdorferi. The detection of this bacterium could be made from any clinical material using the DNA diagnostics in Europaen Union. There can be found three pathogenic species of Borrelia genus: B. b. sensu stricto, B. afzelii and B. garinii. EliGene® Borrelia Kits contain mix of primers and probes that are specific to all three mentioned Borrelia species. DNA diagnostics is useful in cases of Borrelia suspicion (anamnesis of tick or insect that suck the blood). |
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| References: |
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| Hytönen J, Hartiala P, Oksi J, Viljanen MK. 2008. Borreliosis: recent research, diagnosis, and management. Scand J Rheumatol. 37(3):161-172. Priem S, Rittig MG, Kamradt T, Burmester GR, Krause A. 1997. An optimized PCR leads to rapid and highly sensitive detection of Borrelia burgdorferi in patients with Lyme borreliosis. J Clin Microbiol. 35(3): 685–690. |
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| Figure E. Detection of pathogenic Borrelia sp. in DNA samples (isolated from ticks) by EliGene® Borelia RT kit on ABI®7500FAST instrument. |
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| Figure F. Detection of pathogenic Borrelia sp. in DNA samples (isolated from ticks) by EliGene® Borelia RT kit on ABI®7500FAST instrument. |
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